Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.
Identifieur interne : 000948 ( Main/Exploration ); précédent : 000947; suivant : 000949Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.
Auteurs : T. Mozo [Pays-Bas] ; P J HooykaasSource :
- Plant molecular biology [ 0167-4412 ] ; 1992.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Agrobacterium tumefaciens, Tabac, Virulence.
- pathogénicité : Agrobacterium tumefaciens.
- Cinétique, Mutation, Plasmides, Transformation génétique, Végétaux toxiques.
English descriptors
- KwdEn :
- MESH :
- genetics : Agrobacterium tumefaciens, Tobacco, Virulence.
- pathogenicity : Agrobacterium tumefaciens.
- Kinetics, Mutation, Plants, Toxic, Plasmids, Transformation, Genetic.
Abstract
Different factors involved in the early steps of the T-DNA transfer process were studied by using a beta-glucuronidase gene (gusA) as a reporter in Nicotiana glauca leaf disc transformation experiments. The levels of transient expression of the gusA gene in leaf discs infected with several strains or vir mutants correlated well with their virulence phenotype, except for virC mutants. The rate of T-DNA transfer was shown to be stimulated in the case of non-oncogenic strains by the co-transfer of small amounts of oncogenic genes. It was found that the location of the T-DNA in the Agrobacterium genome affected the T-DNA transfer rate especially in virC mutants. The virC mutants transferred the gusA-containing T-DNA located on a binary vector more efficiently than the oncogenic T-DNA of the Ti plasmid. Although wild-type strains induced high levels of gusA expression early after infection, the gusA expression appeared to be lost late after infection in the infected leaf discs. In contrast, in leaf discs infected by virC mutants the level of gusA expression increased steadily in time. A model explaining these results is presented.
DOI: 10.1007/BF00040533
PubMed: 1511127
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.</title><author><name sortKey="Mozo, T" sort="Mozo, T" uniqKey="Mozo T" first="T" last="Mozo">T. Mozo</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University</wicri:regionArea><wicri:noRegion>Leiden University</wicri:noRegion></affiliation></author><author><name sortKey="Hooykaas, P J" sort="Hooykaas, P J" uniqKey="Hooykaas P" first="P J" last="Hooykaas">P J Hooykaas</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1511127</idno><idno type="pmid">1511127</idno><idno type="doi">10.1007/BF00040533</idno><idno type="wicri:Area/Main/Corpus">000947</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000947</idno><idno type="wicri:Area/Main/Curation">000947</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000947</idno><idno type="wicri:Area/Main/Exploration">000947</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.</title><author><name sortKey="Mozo, T" sort="Mozo, T" uniqKey="Mozo T" first="T" last="Mozo">T. Mozo</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University</wicri:regionArea><wicri:noRegion>Leiden University</wicri:noRegion></affiliation></author><author><name sortKey="Hooykaas, P J" sort="Hooykaas, P J" uniqKey="Hooykaas P" first="P J" last="Hooykaas">P J Hooykaas</name></author></analytic><series><title level="j">Plant molecular biology</title><idno type="ISSN">0167-4412</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Agrobacterium tumefaciens (pathogenicity)</term><term>Kinetics (MeSH)</term><term>Mutation (MeSH)</term><term>Plants, Toxic (MeSH)</term><term>Plasmids (MeSH)</term><term>Tobacco (genetics)</term><term>Transformation, Genetic (MeSH)</term><term>Virulence (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (pathogénicité)</term><term>Cinétique (MeSH)</term><term>Mutation (MeSH)</term><term>Plasmides (MeSH)</term><term>Tabac (génétique)</term><term>Transformation génétique (MeSH)</term><term>Virulence (génétique)</term><term>Végétaux toxiques (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Tobacco</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Tabac</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Kinetics</term><term>Mutation</term><term>Plants, Toxic</term><term>Plasmids</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Mutation</term><term>Plasmides</term><term>Transformation génétique</term><term>Végétaux toxiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Different factors involved in the early steps of the T-DNA transfer process were studied by using a beta-glucuronidase gene (gusA) as a reporter in Nicotiana glauca leaf disc transformation experiments. The levels of transient expression of the gusA gene in leaf discs infected with several strains or vir mutants correlated well with their virulence phenotype, except for virC mutants. The rate of T-DNA transfer was shown to be stimulated in the case of non-oncogenic strains by the co-transfer of small amounts of oncogenic genes. It was found that the location of the T-DNA in the Agrobacterium genome affected the T-DNA transfer rate especially in virC mutants. The virC mutants transferred the gusA-containing T-DNA located on a binary vector more efficiently than the oncogenic T-DNA of the Ti plasmid. Although wild-type strains induced high levels of gusA expression early after infection, the gusA expression appeared to be lost late after infection in the infected leaf discs. In contrast, in leaf discs infected by virC mutants the level of gusA expression increased steadily in time. A model explaining these results is presented.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1511127</PMID><DateCompleted><Year>1992</Year><Month>09</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0167-4412</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>6</Issue><PubDate><Year>1992</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Plant molecular biology</Title><ISOAbbreviation>Plant Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.</ArticleTitle><Pagination><MedlinePgn>1019-30</MedlinePgn></Pagination><Abstract><AbstractText>Different factors involved in the early steps of the T-DNA transfer process were studied by using a beta-glucuronidase gene (gusA) as a reporter in Nicotiana glauca leaf disc transformation experiments. The levels of transient expression of the gusA gene in leaf discs infected with several strains or vir mutants correlated well with their virulence phenotype, except for virC mutants. The rate of T-DNA transfer was shown to be stimulated in the case of non-oncogenic strains by the co-transfer of small amounts of oncogenic genes. It was found that the location of the T-DNA in the Agrobacterium genome affected the T-DNA transfer rate especially in virC mutants. The virC mutants transferred the gusA-containing T-DNA located on a binary vector more efficiently than the oncogenic T-DNA of the Ti plasmid. Although wild-type strains induced high levels of gusA expression early after infection, the gusA expression appeared to be lost late after infection in the infected leaf discs. In contrast, in leaf discs infected by virC mutants the level of gusA expression increased steadily in time. A model explaining these results is presented.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mozo</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hooykaas</LastName><ForeName>P J</ForeName><Initials>PJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Plant Mol Biol</MedlineTA><NlmUniqueID>9106343</NlmUniqueID><ISSNLinking>0167-4412</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><GeneSymbolList><GeneSymbol>vir</GeneSymbol></GeneSymbolList><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010947" MajorTopicYN="Y">Plants, Toxic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="Y">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>9</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1511127</ArticleId><ArticleId IdType="doi">10.1007/BF00040533</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nucleic Acids Res. 1988 May 25;16(10):4621-36</Citation><ArticleIdList><ArticleId IdType="pubmed">2837739</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1990 Feb;14(2):249-59</Citation><ArticleIdList><ArticleId IdType="pubmed">2101693</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51</Citation><ArticleIdList><ArticleId IdType="pubmed">7012838</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1986 Aug 1;46(3):325-33</Citation><ArticleIdList><ArticleId IdType="pubmed">3731272</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1983;2(3):403-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11894956</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1990 Dec;15(6):851-64</Citation><ArticleIdList><ArticleId IdType="pubmed">2103477</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1983;2(12):2143-50</Citation><ArticleIdList><ArticleId IdType="pubmed">16453482</ArticleId></ArticleIdList></Reference><Reference><Citation>Plasmid. 1984 Sep;12(2):139-41</Citation><ArticleIdList><ArticleId IdType="pubmed">6095352</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1986 Apr;83(8):2571-5</Citation><ArticleIdList><ArticleId IdType="pubmed">3458219</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1990 Apr;2(4):279-289</Citation><ArticleIdList><ArticleId IdType="pubmed">12354959</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1986 Jul;5(7):1445-54</Citation><ArticleIdList><ArticleId IdType="pubmed">3017694</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Dec;86(23):9154-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2556703</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1987 Nov 11;15(21):8983-97</Citation><ArticleIdList><ArticleId IdType="pubmed">3684577</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1990 Apr;2(4):291-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2152117</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1988 Sep 20;203(2):373-84</Citation><ArticleIdList><ArticleId IdType="pubmed">3199438</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1989 Dec;171(12):6845-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2592351</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1991 Jan;9(9):479-83</Citation><ArticleIdList><ArticleId IdType="pubmed">24213784</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Feb;86(4):1193-7</Citation><ArticleIdList><ArticleId IdType="pubmed">2919168</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1991 May;16(5):917-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1859872</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1991 Aug;228(1-2):104-12</Citation><ArticleIdList><ArticleId IdType="pubmed">1886604</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1986 Oct;168(1):244-50</Citation><ArticleIdList><ArticleId IdType="pubmed">3759904</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1980 Sep;143(3):1295-306</Citation><ArticleIdList><ArticleId IdType="pubmed">7410319</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1980 Nov;144(2):732-43</Citation><ArticleIdList><ArticleId IdType="pubmed">6253441</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1986 Apr;5(2):81-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24248039</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1984 Nov 26;12(22):8711-21</Citation><ArticleIdList><ArticleId IdType="pubmed">6095209</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1987 Mar;169(3):1046-55</Citation><ArticleIdList><ArticleId IdType="pubmed">3029014</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1989 Mar;8(3):643-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16453872</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1990 Jul;222(2-3):329-36</Citation><ArticleIdList><ArticleId IdType="pubmed">1703268</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1984 May;158(2):754-6</Citation><ArticleIdList><ArticleId IdType="pubmed">6373732</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1990 Jan;220(2):245-50</Citation><ArticleIdList><ArticleId IdType="pubmed">2325623</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1986 May;83(10):3282-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16593697</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1986;41(2-3):337-42</Citation><ArticleIdList><ArticleId IdType="pubmed">3011607</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1989 Mar-Apr;2(2):43-52</Citation><ArticleIdList><ArticleId IdType="pubmed">2520160</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1985 Mar 8;227(4691):1229-31</Citation><ArticleIdList><ArticleId IdType="pubmed">17757866</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1983 Jul;80(14):4281-5</Citation><ArticleIdList><ArticleId IdType="pubmed">6410388</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1990 Jan;14(1):61-72</Citation><ArticleIdList><ArticleId IdType="pubmed">2101312</ArticleId></ArticleIdList></Reference><Reference><Citation>Plasmid. 1984 May;11(3):195-205</Citation><ArticleIdList><ArticleId IdType="pubmed">6087390</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1989 Jan 27;56(2):193-201</Citation><ArticleIdList><ArticleId IdType="pubmed">2643473</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1986 Jun;83(11):3895-9</Citation><ArticleIdList><ArticleId IdType="pubmed">3459162</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1986 Jun;5(6):1137-42</Citation><ArticleIdList><ArticleId IdType="pubmed">15966101</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Hooykaas, P J" sort="Hooykaas, P J" uniqKey="Hooykaas P" first="P J" last="Hooykaas">P J Hooykaas</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Mozo, T" sort="Mozo, T" uniqKey="Mozo T" first="T" last="Mozo">T. Mozo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000948 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000948 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:1511127
|texte= Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:1511127" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |